T7 Exonuclease
Cat.No：Q3020 Solarbio
Storage：Store at -20℃，3 years.
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Molecular Cloning-Related Enzymes > Exonuclease > T7 Exonuclease
Product Information
| Name | T7 Exonuclease |
| Storage | Store at -20℃，3 years. |
| Unit | Bottle |
| Specification | 1000U |
Product Introduction
T7 exonuclease acts on double-stranded DNA and catalyzes the removal of 5′ mononucleotides along the 5′→3′ direction. It can start digestion from the end of 5′ or from the cut or gap of double-stranded DNA. It can degrade both 5′ phosphorylated DNA
Also degrades 5′ dephosphorylated DNA. It has been reported to degrade RNA or DNA in the 5′→3′ direction of the RNA/DNA hybrid double strand, but not double - or single-stranded RNA.
Precautions
(1) 1×T7ExoBuffer: 50mMKAc, 20mMTris-Ac, 10mM
Mg(Ac) 2,1 mMDTT (pH7.9@25℃), incubated at 25℃.
(2) The optimal reaction temperature of the enzyme is 25℃, and it can be inactivated for 20min at 75℃.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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