Products
T5 Exonuclease

Storage:Store at -20℃,3 years.

T5 Exonuclease
Cat.No:
Q3030
Brand:
Solarbio

Product Introduction

T5 exonuclease degrades DNA in the 5 '-3' direction, and it can degrade double-stranded DNA, single-stranded DNA and engraved plasmid DNA. It can degrade DNA both from the 5 '-terminal and from incisions or gaps in linear or circular double-stranded DNA, but cannot degrade superhelical double-stranded DNA. Based on the above characteristics, T5 exonuclease can be applied to Gibson assembly.

Precautions

(1) 1 x T5ExoBuffer: 50mMKAc, 20 mmtrIS-ACph7.9, 10 MMMG (Ac) 2,1 mMDTT. This enzyme is also active in the PCRBuffer.

(2) The optimal reaction temperature of the enzyme is 37℃, and it is also active at 50℃, so it can be assembled by Gibson.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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