Product introduction

1. Product name: Yeast defective medium SD-Trp-Leu-His-Ade

2. SD-Trp-Leu-His-Ade

3. Product properties: this product is white powder, odorless or slightly smelly

4. solubility: distilled water, 8g/L concentration

Yeast is a common model organism and is often used for genetic modification. Yeast amino acid deficient medium is necessary for yeast transformation and yeast hybridization. Its main uses include yeast single/yeast two-hybrid experimental research and yeast genetic mutant screening. According to the type of defective amino acids, it is divided into four types of media, namely one deficient, two deficient, three deficient, four deficient media, the company can customize the relevant media according to your requirements, quality assurance.

4-deficient medium: This type of yeast SC selective medium -SD medium is mainly used for reporter gene analysis.

Uses: yeast two-hybrid system and yeast three-hybrid system reporter gene detection, Y187 and AH109 yeast after mating experiment reporter gene analysis (His3, Ade2)

For example: SD/-Trp-Leu-His-Ade, SD/-Ura-Trp-Leu-His, SD/-Ura-Trp-Leu-Met, SC-Trp-Leu-His-Ade, SC-Ura-Trp-Leu-His, SC-Ura-Trp-Leu-Met

Precautions

1: The medium contained all the desired ingredients (except glucose, since some researchers need to use other sugars such as galactose to induce GAL1 promoter expression), a fine mixture of various amino acids and yeast nitrogen base/yeast nitrogen source, weighed at 8g/L, and added to water to make the corresponding medium. There is no need to purchase MinimalSDBase or other yeast nitrogen components. Autoclaving with sterile glucose to a final concentration of 2% (or raffinose) or the specific inducer required for the experiment (such as galactose used in the GAL1 promoter to induce expression at a dose or concentration of 2%) is suitable for the expression of foreign genes in yeast. Selective deficient cultures such as yeast yeastone-Hybrid and yeasttwo-Hybrid systems are used to screen for yeast phenotypes containing specific marker genes.

2: When yeast deficient medium is prepared, glucose can be added first followed by high pressure without serious effect on yeast growth. However, if glucose is added and then sterilized by high pressure, the medium will change color, ranging from light yellow to dark brown, which still has a certain effect on the growth of cells (but will not affect the experiment in general). Recommendation: Because amino acids and GLUCOSE can react chemically at high temperature and pressure, the Technical department does not recommend adding glucose to the post pressure, but recommends the method of the above tip 1.

3 Recommendation: raffinose and galactose cannot be exposed to high temperature and high pressure in principle, because their chemical structure will change under high temperature and high pressure, which will inhibit the induction effect. This point is especially important for researchers to pay attention to, especially when performing functional complementation experiments mediated by expression regulation.

4: When the plate is prepared, the soft Agar semi-solid medium (Agar powder is 2%, that is, 2 grams of Agar powder per 100ml) is used for transformation screening, the pH needs to be adjusted to about 6.0 before high pressure (for convenience, the pH range can be changed between 5.6 and 6.5), while the liquid medium does not need to be adjusted.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.