Pinocembrin

Examples of using this product（for reference only）

HPLC-ESI-QTOF-MS/MS

The liquid chromatography analyses were performed on a HPLC system. Chromatographic separation was implemented on an C18 column with detection being carried out at 280 nm with the operating temperatures being kept at 35℃. The analyses were completed with a gradient elution of methol (A) and 0.1% formic acid in purified water (B). The gradient protocol was: 22–36% A, 0–5 min; 36–52% A, 5–30 min; 52–63% A, 30–65 min; 63–70% A, 65–95 min; 70–80% A, 95–120 min; 80–22% A, 120–122 min. The injected sample volume was 5 μL, and the flow rate was 0.6 mL/min. An electrospray ionization source was used to drive the TOF mass spectrometer. The capillary voltage was set to 4 kV; the collision voltage was set to 135 V; the drying-gas temperature was set to 350 ℃; the dryinggas flow rate was set to 10 L/min; the nebulizer pressure was set to 40 psi; the collision gas was nitrogen; the collision energy was set to 30 eV; the full ionic scan mode was used; and the scan range was set to m/z = 50–2000.

References：

Xu W, Lu H, Yuan Y, Deng Z, Zheng L, Li H. The Antioxidant and Anti-Inflammatory Effects of Flavonoids from Propolis via Nrf2 and NF-κB Pathways. Foods. 2022 Aug 13;11(16):2439. doi: 10.3390/foods11162439. PMID: 36010439; PMCID: PMC9407528.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.