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CAS:480-39-7
Appearance:White to off-white powder
Storage:2-8℃
Purity:HPLC≥98%
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HPLC-ESI-QTOF-MS/MS
The liquid chromatography analyses were performed on a HPLC system. Chromatographic separation was implemented on an C18 column with detection being carried out at 280 nm with the operating temperatures being kept at 35℃. The analyses were completed with a gradient elution of methol (A) and 0.1% formic acid in purified water (B). The gradient protocol was: 22–36% A, 0–5 min; 36–52% A, 5–30 min; 52–63% A, 30–65 min; 63–70% A, 65–95 min; 70–80% A, 95–120 min; 80–22% A, 120–122 min. The injected sample volume was 5 μL, and the flow rate was 0.6 mL/min. An electrospray ionization source was used to drive the TOF mass spectrometer. The capillary voltage was set to 4 kV; the collision voltage was set to 135 V; the drying-gas temperature was set to 350 ℃; the dryinggas flow rate was set to 10 L/min; the nebulizer pressure was set to 40 psi; the collision gas was nitrogen; the collision energy was set to 30 eV; the full ionic scan mode was used; and the scan range was set to m/z = 50–2000.
References：
Xu W, Lu H, Yuan Y, Deng Z, Zheng L, Li H. The Antioxidant and Anti-Inflammatory Effects of Flavonoids from Propolis via Nrf2 and NF-κB Pathways. Foods. 2022 Aug 13;11(16):2439. doi: 10.3390/foods11162439. PMID: 36010439; PMCID: PMC9407528.
