Caspase-3 Activity Assay Kit
Cat.No：BC3830 Solarbio
Detection Method：Colorimetric
Storage：Store at -20℃，6 months
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PI3K/Akt/mTOR
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HIF
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Apoptosis
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Hippo
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> Biochemical Assay Kits > Energy Metabolism Series > Caspase Series > Caspase-3 Activity Assay Kit
Product Information
| Name | Caspase-3 Activity Assay Kit |
| Detection Method | Colorimetric |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 20T 50T 100T |
Determination of Significance：
Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-3 is the most important terminal protease in the process of apoptosis, and it is also the most studied caspase; it activates pro-caspase-2,6,7,9, specifically hydrolyzes a variety of key apoptotic proteins, such as PARP, and mediates chromatin condensation, apoptotic body formation and nuclear DNA fragmentation.
Measurement Principle：
The caspase-3 colorimetric assay is based on the hydrolysis of the peptide substrate DEVD-pNA (Asp-Glu-Val-Asp-p-nitroanilide) by caspase-3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.
Self Provided：
Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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