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Caspase-4 Activity Assay Kit

Storage:Store at -20℃,6 months

Caspase-4 Activity Assay Kit
Cat.No:
BC3840
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Determination of Significance:

Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-4 can interact with Apaf-1, participate in the activation of Caspase-3 induced by cytochrome c, and also interact with caspase-14.

Measurement Principle:

The caspase-4 colorimetric assay is based on the hydrolysis of the peptide substrate Ac-LEVD-pNA by caspase-4, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.

Self Provided:

Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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