Determination of Significance:
Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-6, also known as Mch-2, its precursor protein is cleaved by granzyme B to form activated caspase-6 dimer, which can induce apoptosis. Caspase-6 can cleave PARP, a key regulator of apoptosis, and Lamin A, a key protein on the nuclear membrane. Its recognition site for Lamin A is VEID.
Measurement Principle:
The caspase-6 colorimetric assay is based on the hydrolysis of the peptide substrate Ac-VETD-pNA (N-acetyl-Val-Glu-Ile-Asp-p-nitroanilide) by caspase-6, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.
Self Provided:
Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water
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