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Caspase-9 Activity Assay Kit

BC3890-241212Manual

Cat.No：BC3890 Solarbio

Detection Method：Colorimetric

Storage：Store at -20℃，6 months

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Product Information

Name	Caspase-9 Activity Assay Kit
Detection Method	Colorimetric
Storage	Store at -20℃，6 months
Unit	Kit
Specification	20T 50T 100T

Determination of Significance：

Caspase is a family of proteases involved in the process of cell apoptosis, including more than 10 members. Caspase-9, also known as ICE-LAP6 or Mch6, can form a complex with cytochrome c and Apaf1 and to be activated, further activate caspase-3, the most critical enzyme for cell apoptosis, thereby triggering the apoptosis cascade. Caspase-9 is an important upstream caspase in the process of apoptosis signal transduction, and its activation can be regulated by phosphorylation.

Measurement Principle：

The caspase-9 colorimetric assay is based on the hydrolysis of the peptide substrate acetyl- Leu-Glu-His-Asp-p-nitroanilide (LEHD-pNA) by caspase-9, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.

Self Provided：

Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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