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CAS:77-95-2
Purity:HPLC≥98%
Appearance:White to off-white powder
Storage:Store at 2-8℃，2 years.
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HPLC
Using HPLC equipped with a C18 250 mm × 4.6 mm, 5 μm column. The mobile phase was ultrapure water containing 0.23% (m/v) Ammonium dihydrogen phosphate. UV detection at a wavelength of 210 nm and a flow rate of 0.8 mL/min were used for the analysis of organic acids.
References：
Zhang X, Li Y, Li Y, Zhao J, Cheng Y, Wang Y, Guan J. Changes of Bioactive Components and Antioxidant Capacity of Pear Ferment in Simulated Gastrointestinal Digestion In Vitro. Foods. 2023 Mar 13;12(6):1211. doi: 10.3390/foods12061211. PMID: 36981138; PMCID: PMC10048753.
HPLC
HPLC was performed using an equipped with a DAD detector and a C18 (250 mm × 4.6 mm; 5 μm). The mobile phase included 0.2% phosphoric acid plus 0.4% sodium 1-heptanesulfonate (A) and methanol (B) at a flow speed of 1.0 ml/min in the condition of a column temperature of 30℃. The detection wavelength was set at 214 nm. The reference standard sample injection volume was 10 μl. The gradient elution was as follows: 0–5 min (100–100% A, 0%–0% B), 5–60 min (100–60% A, 0%–40% B), 60–120 min (60–30% A, 40–70% B), 120–125 min (30–30% A, 70–70% B), 125–125.1 min (30–100% A, 70–0% B), 125.1–132 min (100–100% A, 0–0% B). The chromatographic data and peak area scores were collected and analyzed using ChemStation software.
References：
Duan Q, Liu T, Huang C, Shao Q, Ma Y, Wang W, Liu T, Sun J, Fang J, Huang G, Chen Z. The Chinese Herbal Prescription JieZe-1 Inhibits Membrane Fusion and the Toll-like Receptor Signaling Pathway in a Genital Herpes Mouse Model. Front Pharmacol. 2021 Sep 24;12:707695. doi: 10.3389/fphar.2021.707695. PMID: 34630083; PMCID: PMC8497740.
