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CAS:529-53-3
Purity:HPLC≥98%
Appearance:Yellow to brown powder
Storage:Store at 2-8℃，2 years.
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HPLC
Apigenin, scutellarein, scutellarin, and apigenin-7-O-glucuronide were measured at 335 nm using a Kinetex 5 μm Biphenyl 100 ? LC Column (250 × 4.6 mm; Phenomenex, USA) operating at 30 ℃. The mobile phase consisted of 0.1% formic acid and acetonitrile with methanol at a flow rate of 1 ml min?1 using the following gradients: 0–20 min, 22% acetonitrile, 5% methanol; 20–22 min, acetonitrile increased from 22 to 90%; 25 min, 22% acetonitrile. Subsequently, the column was washed and equilibrated for 5 min before the next injection. Thirty microliters of the sample were injected into the HPLC system and each run was stopped at 30 min after the injection.
References：
Liu X, Cheng J, Zhang G, Ding W, Duan L, Yang J, Kui L, Cheng X, Ruan J, Fan W, Chen J, Long G, Zhao Y, Cai J, Wang W, Ma Y, Dong Y, Yang S, Jiang H. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches. Nat Commun. 2018 Jan 31;9(1):448. doi: 10.1038/s41467-018-02883-z. PMID: 29386648; PMCID: PMC5792594.
