D(+)-Sucrose

Examples of using this product（for reference only）

UV

A fruit sample of approximately 1.0 g was placed in a prechilled mortar, ground into a homogenate with 80% (v/v) ethanol on an ice bath and transferred into a stoppered test tube. Then, 9 mL of 80% (v/v) ethanol was added, and the mixture was boiled in a boiling water bath for 30 min. Afterwards, it was removed and cooled in ice water, then it was centrifuged at a low temperature of 8000 r/min for 20 min, and the supernatant was discarded. An amount of 9 mL of 80% (v/v) ethanol was added again and the above steps were repeated once. The residue was dried, then 2 mL of distilled water was added. It was boiled in a boiling water bath for 15 min and cooled in ice-cold water. Then, 2 mL of cold 9.2 mol/L perchloric acid (HClO4) was added for 15 min, followed by an addition of 6 mL of distilled water, mixing and centrifuging at a low temperature of 8000 r/min for 10 min, and the transference of the supernatant to a 25 mL volumetric flask. An amount of 2 mL of cold 4.6 mol/L HClO4 was added to the filter residue for 15 min, then 6 mL of distilled water was added, followed by centrifugation for 20 min and the transference of the supernatant to a 25 mL volumetric flask. The precipitate was washed twice with 2 mL of distilled water, followed by centrifugation and the transference of the supernatant to the volumetric flask. Finally, the volumetric flask was filled with distilled water to 25 mL, resulting in the starch extraction solution. The detection of starch was performed at 620 nm with a visible ultraviolet spectrophotometer UV.

References：

Han W, Wang Y, Li H, Diao S, Suo Y, Li T, Sun P, Li F, Fu J. Transcriptome and Metabolome Reveal Distinct Sugar Accumulation Pattern between PCNA and PCA Mature Persimmon Fruit. Int J Mol Sci. 2023 May 11;24(10):8599. doi: 10.3390/ijms24108599. PMID: 37239943; PMCID: PMC10217969.

TPS-MFGDP-MS

The conditions of mass spectrometer were optimized as follows: capillary voltage, 18 V; capillary temperature, 320 ℃; tube lens, 20 V. All spectra were collected under automatic gain mode where per spectrum was set 3 microscans at 100 ms maximum ion injection time. The flow of He through the channel was controlled by a mass flow controller (D08-1F3L, Sevenstar, Beijing, China) at a rate of 1 mL min ?1.

References：

Li Y, Guo X, Tian Y, Zhang T, Luo Z, Liu X, Qian C, Dai J, Duan Y. Methylation in combination with temperature programming enables rapid identification of polysaccharides by ambient micro-fabrication glow discharge plasma (MFGDP) desorption ionization mass spectrometry. Talanta. 2020 Oct 1;218:121156. doi: 10.1016/j.talanta.2020.121156. Epub 2020 May 15. PMID: 32797915.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.