Product introduction

Chemical examination of stool includes pH reaction, occult blood test, determination of bile pigment and fat, among which occult blood test has the most important clinical application value. The amount of bleeding in the upper gastrointestinal tract is less than 5ml, there is no visible blood in the stool, and the red blood cells are destroyed, and no red blood cells are found in the microscopic examination. Bleeding that needs to be confirmed by chemical methods, immunoassay, transferrin method, and hemoglobin fluorescence assay is called occult blood. The test to detect fecal occult blood is called fecal occult blood test (fecal occult blood test, FOBT). Chemical method commonly used reagents are o-toluidine, pitami hole, guaiac, colorless malachite green, mitul, etc.

The detection principle of the kit is that the heme in hemoglobin has a peroxidase-like activity, which can catalyse H2O2 as an electron acceptor to oxidize the chromogenic substrate o-toluidine to o-methazobene, which turns green to blue, and its color is proportional to the hemoglobin content. According to the speed of color development, the hemoglobin, that is, the occult blood content, can be semi-quantitative. Therefore, it is also called fecal occult blood semi-quantitative test, which is mostly used for human or animal fecal hemoglobin examination. It should be noted that normal human or animal digestive tract bleeding or bleeding after stimulation, or model animal feed containing fish meal, iron, etc., may lead to false positive test results. This method is characterized by high sensitivity and high false positive. It can detect Hb from 0.2 μg/ml to 1.0μg/ml, and can be used in combination with Pilamidonne method with medium sensitivity and specificity.

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