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My Cart> Biochemical Assay Kits > Other Biochemical Series > Other Series > Calcium Test Kit (methyl thymol Blue Microplate method)
Detection Method：Methyl Thymol Blue Microplate Method
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| Detection Method | Methyl Thymol Blue Microplate Method |
| Unit | Kit |
| Specification | 100T |
Product introduction
Calcium (Calcium) is a metallic element, at room temperature as a silver-white crystal, animal bones, clam shells, eggshells contain calcium carbonate. The detection of calcium content in living body is mainly through the detection of calcium ion concentration, which is commonly detected by spectrophotometry. This method requires suitable metal indicators or dye compounds that selectively combine with calcium ions and cause color change. At present, the commonly used dyes include o-cresolphthalide complex ketone (OCPC), azo arsenic Ⅲ, methyl thymol blue (MTB), etc.
The kit uses calcium ions in solution to combine with MTB under alkaline conditions to form a navy/blue complex. Magnesium ion chelator is added to remove magnesium ion background interference. The absorbance at 610nm is detected by microplate detector, and the total calcium content is calculated according to the formula.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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