Golgi protein extraction kit

Golgi apparatus (Golgi bodies) are organelles composed of many flat vesicles whose main function is secretion. Also known as Golgi apparatus or Golgi complex; The Golgi apparatus is a highly polar organelle formed by several flat vesicles stacked together. It is usually distributed between the endoplasmic reticulum and the cell membrane, and is arcuated or hemispherical, with the convex side facing the endoplasmic reticulum called the formingface or cis face. The concave side facing the plasma membrane is called the mature face or the trans face. There are some large or small transport vesicles on both the front and back sides. In polar cells, Golgi bodies are often distributed in large quantities in the cytoplasm of the secretory end. Because it looks very similar to the smooth endoplasmic reticulum, some scientists believe that it evolved from the smooth endoplasmic reticulum. The main enzymes in the Golgi apparatus are glycosyltransferase, sulfo-glycosyltransferase, oxidoreductase, phosphatase, protein kinase, mannosidase, transferase and phospholipase. The main function of the Golgi apparatus is to process, classify, and package the proteins synthesized by the endoplasmic reticulum, and then send them to specific parts of the cell or secrete them outside the cell. This kit contains protease inhibitor mixture, protein phosphatase inhibitor mixture, etc., which prevents protease from degrading protein and provides a guarantee for extracting high-purity protein. The extracted protein can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, and immunoprecipitation.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.