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Storage:4℃, avoid light and freeze.
Ex/Em: 651/662 nm
Phycobilin is composed of many subunits, each of which has a protein skeleton to which the linear tetrapyrrole chromophore is covalently bound. Phycoglobin (red) and phycocyanin (blue) are the two main phycobilin proteins. Phycoglobin (PE) has a maximum absorption between 490 and 570 nm, while phycocyanin (PC) has a maximum absorption between 610 and 665 nm. In general, phycobilin has good long-term stability when stored in cold storage in a solution containing ammonium sulfate. Purified phycobilin may decompose into subunits under acidic or alkaline conditions, but is relatively stable at room temperature and in neutral solutions at concentrations greater than 0.1 mg/ml. The dissociated subunits generally have lower coloration and fluorescence than the natural pigments. It is recommended that all phycobilin and its conjugations (preferably in a neutral buffer solution) be stored refrigerated and not frozen.
Phycobilin proteins (including B-phycoglobin (B-PE), R-phycoglobin (R-PE), and allophycocyanin (APC)) are super-sensitive fluorescent dyes for biological detection. They are more than 100 times more sensitive than conventional organic fluorophores, even in practical applications such as flow cytometry and immunoassay. The sensitivity of phycobilin-bound antibodies is usually much higher than that of corresponding organic antibodies based on molecular conjugates.
Allophycocyanin (APC) is a phycobilin isolated from spirulina, a blue-green algae. Like other phycobilic proteins, APC is a fluorescent substance with extremely high absorption rate and high quantum efficiency. It is a protein that can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without changing its spectral signature. Allophycocyanin is the most unstable of the major phycobilis proteins and readily dissociates at low concentrations, including some experimental concentrations. CL-APC refers to the chemical cross-linking of α and β subunits to form a more stable structure than APC. The cross-linked allophycocyanin showed improved stability in aqueous solution.
Note:
1. The product is in solution form, and the solution is a neutral buffer containing ammonium sulfate.
2. Keep the product at 4℃ and avoid freezing.
3. Fluorescent dyes have quenching problems, please try to avoid light to slow down fluorescence quenching.
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