During cell apoptosis, a number of endonuclides are activated, which cut the genomic DNA between nucleosomes, producing 180 bp-200 bp DNA fragments, which are represented by the specific Ladder pattern shown in agarose gel electrophoresis. When the double or single strand of genomic DNA breaks, a large number of sticky 3 '-OH ends are produced, which can be catalyzed by deoxyribonucleotide end transferase (TdT) and bind to biotin-DUTP, thus directly detecting apoptotic cells through optical microscopy. Such methods are called Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). Since normal or proliferating cells have few breaks in DNA, no 3 '-OH is formed and can rarely be stained. Tunel method can stain a single apoptotic nucleus or apoptotic body in situ, accurately reflect the typical biochemical and morphological characteristics of apoptosis, and can detect a very small number of apoptotic cells, so it is widely used in the study of apoptosis. This kit has a wide range of applications and can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent cells or suspended cells. It can selectively detect apoptotic cells, but not dead cells or cells with broken DNA strands caused by radiation and drug treatment.

Precautions

1. Please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments.

2. Sodium azide can inhibit HRP, do not use reagents containing sodium azide in the experiment.

3. Wear A mask and gloves when using components A, E, F and G. In case of contact with skin, rinse immediately with plenty of water.

4. For your safety and health, please wear a lab coat and disposable gloves.

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