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Cy3 TUNEL apoptosis kit

Storage:Store at -20℃, avoid light and freeze/thaw cycles for the component A.

Cy3 TUNEL apoptosis kit
Cat.No:
T2192
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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细胞发生凋亡时, 会激活一些DNA内切酶,这些内切酶会切断核小体间的基因组DNA,产生180 bp-200 bp的 DNA 片段,表现为琼脂糖凝胶电泳中呈现的特异的梯状Ladder图谱。基因组DNA双链或单链断裂时会出现产生大量的粘性3'-OH末端,可在脱氧核糖核苷酸末端转移酶(TdT)的催化作用下,与SF/Cy-dUTP结合,从而通过荧光显微镜或流式细胞仪直接进行凋亡细胞的检测,这类方法称为脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(Terminal -deoxynucleotidyl transferase mediated nick end labeling, TUNEL)。由于正常的或正在增殖的细胞几乎没有DNA的断裂,因而没有3'-OH形成,很少能够被染色。Tunel法可以对完整的单个凋亡细胞核或凋亡小体进行原位染色,能准确地反应细胞凋亡典型的生物化学和形态特征,可检测出极少量的凋亡细胞,因而在细胞凋亡的研究中广泛采用。本试剂盒应用范围广,可用于检测冷冻或石蜡切片中的细胞凋亡情况,也可检测培养的贴壁细胞或悬浮细胞的凋亡情况。可选择性的检测凋亡细胞,而非坏死细胞或因辐照和药物治疗而造成的 DNA 链断裂的细胞。本试剂盒检测细胞凋亡,耗时短,只需一步染色反应,洗涤后即可检测。

注意事项

1.使用前请将产品瞬时离心至管底,再进行后续实验。

2.染色背景较重或非特异性着色明显时可适当减少染色时间。

3.实验时建议增加阴性对照和阳性对照组。

4.组分A使用时请佩戴口罩、手套,如接触皮肤,请立即用大量水冲洗。

5.荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。

6.为了您的安全和健康,请穿实验服并戴一次性手套操作。


Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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