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α-Ketoglutaric Acid (α-KG) Ccontent Assay kit

BC5425-241212Manual

Cat.No：BC5425 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	α-Ketoglutaric Acid (α-KG) Ccontent Assay kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Alpha-ketoglutarate (α-KG) is an important metabolic intermediate in the tricarboxylic acid cycle and a key link between intracellular carbon and nitrogen metabolism. α-KG, as a short-chain carboxylic acid molecule, is a precursor to many important amino acids such as glutamine and glutamate, and is not only directly involved in energy supply, but also participates in a variety of intracellular chemical reactions and has a variety of physiological effects.

Measurement Principle：

GDH catalyses the formation of glutamate and NAD+ from NH4+, α-ketoglutarate and NADH, causing a decrease in absorbance at 340nm. The α-ketoglutarate content was calculated by measuring the change in NADH.

Self Provided：

Ultraviolet spectrophotometer/ microplate reader, centrifuge, water bath/ heating dry baths / constant temperature incubator, analytical balance, adjustable pipette, micro quartz cuvettes/96-well UV plates, mortar/homogeniser/cell ultrasonicator, distilled water and ice.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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