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Determination of Significance：
Alpha-ketoglutarate (α-KG) is an important metabolic intermediate in the tricarboxylic acid cycle and a key link between intracellular carbon and nitrogen metabolism. α-KG, as a short-chain carboxylic acid molecule, is a precursor to many important amino acids such as glutamine and glutamate, and is not only directly involved in energy supply, but also participates in a variety of intracellular chemical reactions and has a variety of physiological effects.
Measurement Principle：
GDH catalyses the formation of glutamate and NAD+ from NH4+, α-ketoglutarate and NADH, causing a decrease in absorbance at 340nm. The α-ketoglutarate content was calculated by measuring the change in NADH.
Self Provided：
Ultraviolet spectrophotometer/ microplate reader, centrifuge, water bath/ heating dry baths / constant temperature incubator, analytical balance, adjustable pipette, micro quartz cuvettes/96-well UV plates, mortar/homogeniser/cell ultrasonicator, distilled water and ice.
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