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Acetolactate Synthase（ALS）Activity Assay Kit

BC5525-241212Manual

Cat.No：BC5525 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Acetolactate Synthase（ALS）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/24S 100T/48S

Determination of Significance：

Acetolactate Synthase (ALS, EC 4.1.3.18), also known as Acetohydroxyacid synthase (AHAS). ALS is present in plant growth and is a key enzyme in the induction of the biosynthesis of the branched-chain amino acids (Ile, Leu, and Val) during the The key enzyme in the first stage of biosynthesis of branched chain amino acids (Ile, Leu, Val).

Measurement Principle：

ALS catalyzes the formation of acetolactate from pyruvate, which is decarboxylated under certain conditions to form acetoin, which reacts with creatine and α-naphthol under alkaline conditions to form a red substance with a maximum absorbance value at 525 nm.

Self Provided：

Microplate Reader/spectrophotometer, water bath/incubator, benchtop centrifuge, balance, adjustable pipettes, micro glass cuvettes/96-well plates, mortar and homogenizer, distilled water, ice, anhydrous ethanol and ammonium sulphate.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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