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Determination of Significance：
Angiotensin Converting Enzyme (ACE, EC 3.4.15.1, also called ACE1) is a zinc-containing peptidyl dipeptide hydrolase with a calculated molecular mass of 120-150 kDa. ACE is widely detected in the endothelial cell of organ tissues included lung, brain, kidney. ACE catalyzes the formation of angiotensin I to angiotensin II, which could cause vasoconstriction, promote synthesis and release of adrenocortical hormone aldosterone. ACE inhibitors could reduce the production of angiotensin II and increase bradykinin activity, which is helpful for the treatment of hypertension, cardiac hypertrophy, heart failure and other diseases.
Measurement Principle：
ACE could catalyze FAPGG to form FAP and glycylglycine. ACE inhibitors could reduce the hydrolysis of FAPGG by inhibiting ACE activity. FAPGG has a characteristic absorption peak at 340 nm, and ACE inhibitor activity is calculated by measuring the rate of decrease in absorbance at 340nm.
Self Provided：
Spectrophotometer, desk centrifuge, balance, transferpettor, 1mL quartz cuvette, constant temperature foster box/water-bath, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
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