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Free Hemoglobin ( FHb ) Content Assay Kit

BC5595-241212Manual

Cat.No：BC5595 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at 2-8℃，6 months

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Product Information

Name	Free Hemoglobin ( FHb ) Content Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	100T/96S

Determination of Significance：

Red blood cells rupture and free hemoglobin (FHb) content increases in the plasma when hemolysis occurs in blood vessels. Plasma FHb concentration increases mildly to moderately in patients with autoimmune hemolytic anemia, sickle cell disease or thalassemia. The patients have hematuria symptoms who suffer from paroxysmal nocturnal hemoglobinuria, paroxysmal cold hemoglobinuria or unstable hemoglobin disease. It is important to detect FHb content for diagnosis of hemolytic diseases.

Measurement Principle：

Heme in hemoglobin has the catalytic function of peroxidase analogues. It could catalyze the oxidation of 4-aminoantipyrine and phenyl amines by H₂O₂ to form purple quinones. It has a characteristic absorption peak at 546 nm, and its color depth is directly proportional to FHb content.

Self Provided：

Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, balance, transferpettor, micro glass cuvette/96 well plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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