Determination of Significance:
Glycated hemoglobin (GHb) is formed when a non-enzyme reaction occurs between glucose in the serum and hemoglobin in the erythrocyte. The amount of GHb generated is proportional to the mean blood glucose during the 8–10 weeks before the test. GHb is composed of HbA1a, HbA1b and HbA1c. HbA1c accounts for about 70 % and its structure is relatively stable. GHb content is used as a biomarker for diabetes mellitus control.
Measurement Principle:
The hexose moiety of GHb is converted to 5-hydroxymethylfurfural (5-HMF) by heating at 100℃ in the presence of a weak acid. 5-HMF reacts with Thiobarbituric Acid (TBA) to produce colored compound, which has an absorption peak at 443 nm.
Self Provided:
Spectrophotometer, desk centrifuge, balance, water-bath, transferpettor, 1mL glass cuvette, ice and distilled water.
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