English
中文
CAS:123-03-5
Appearance:White to off-white solid
Storage:Store powder at 2-8℃ for 2 years, in solvent(mother liquid) at -20℃ for 1 month, -80℃ for 6 months.
Purity:≥98%
Manual Download
Examples of using this product（for reference only）
In Vitro:
Cell（MC3T3-E1 cells；10% cetylpyridinium chloride；562 nm）:
MC3T3-E1 cells were seeded in a 12-well plate at a density of 2 ×104/mL, and cultured with osteogenic induction extracts, which contained sample extracts, 10 mM β-glycerophosphate, 0.1 mM dexamethasone (Solarbio, China) and 50 μg/mL vitamin C (Solarbio, China). After 7 days of incubation, cells were lysed using inhibitorfree Western and IP cell lysate and total protein was extracted. The bicinchoninic acid protein assay kit was used to determine the total protein content. The ALP detection kit was applied to detect the ALP activity of MC3T3- E1. The results were expressed in U per mg of total protein. Moreover, MC3T3-E1 cells were stained using an ALP staining kit to evaluate the formation of calcified nodules. Alizarin red (Solarbio, China) was used to stain MC3T3-E1 after 21 days of culturing. Then, mineralized nodules were dissolved using 10% of cetylpyridinium chloride (Solarbio, China) and measured the absorbance at 562 nm.
Reference：
Zhang Z, Liu A, Fan J, Wang M, Dai J, Jin X, Deng H, Wang X, Liang Y, Li H, Zhao Y, Wen P, Li Y. A drug-loaded composite coating to improve osteogenic and antibacterial properties of Zn-1Mg porous scaffolds as biodegradable bone implants. Bioact Mater. 2023 Apr 28;27:488-504. doi: 10.1016/j.bioactmat.2023.04.017. PMID: 37180641; PMCID: PMC10173180.
