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CAS	771-97-1
Chinese Name	一氧化氮NO荧光探针
English Name	DAN
Synonyms	2,3-Diaminonaphthalene;2,3-二氨基萘
Molecular Formula	C₁₀H₁₀N₂
Molecular Weight	158.2
Solubility	Soluble in DMSO ≥5mg/mL(Need ultrasonic)
Storage	Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity	≥98%
Appearance	Solid
MDL	MFCD00004116
SMILES	C1=CC=C2C=C(C(=CC2=C1)N)N
InChIKey	XTBLDMQMUSHDEN-UHFFFAOYSA-N
InChI	InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H，11-12H2
PubChem CID	69872
Background	DAN is a fluorescent probe used to detect nitric oxide (NO) production in living cells and animals. Ex (nm) : 364/Em (nm) : 406
Biological Activity	2，3 Diaminonaphthalene is a highly selective colorimetric and fluorometric reagent for selenium detection and also used for the fluorometric determination of nitrite.
In Vitro	Poly(2，3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection[1]. 2，3-Diaminonaphthalene (DAN) is possibly a carcinogenic reagent[2].
Kinase Experiment	Various concentrations of each drug are incubated for 60 min at 37°C in OS-mL reaction volumes containing PBS (pH 7.4)， 200 μM 2，3-Diaminonaphthalene (DAN) and 40 μM Sp/NO. Spermine/NO will spontaneously decompose at pH7.4 to produce 2 mol of NO and 1 mol of spermine with a half-life of 39 min at 37°C. Following the incubation period， 2.5 mL of 10 mM NaOH is added to each tube to stop the reaction. The NO-dependent N-nitrosation of 2，3-Diaminonaphthalene to yield its highly fluorescent N-nitrosated derivative， 2，3-naphthotriazole， is quantified by measuring the fluorescence of each sample using an excitation wavelength of 375 nm and an emission wavelength of 450nm. The concentration of Triazole is determined using standards of the pure 2，3-naphthotriazole[3].
Data Literature Source	[1]. Tian J，et al. Poly(2，3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection. Analyst. 2011 Jun 7;136(11):2221-4. [2]. Martínez-Tomé MJ，et al. Immobilization and characterization of 2，3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite. J Fluoresc. 2009 Jan;19(1):119-25. [3]. Grisham MB，et al. Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Biochem Pharmacol. 1994 May 18;47(10):1897-902.
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