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Guanidinium isothiocyanate belongs to the uncoupling agent, is a kind of strong protein denaturant, can dissolve proteins, the main role is to lysis cells, make the cells in the protein, nucleic acid material depolymerization release, extract RNA and DNA.
It was used as a strong denaturant in experiments to extract total RNA from cells. Guanidine hydrochloride solution can dissolve proteins, leading to the destruction of cell structure and secondary structure of nuclear protein, which dissociates from nucleic acid. It is also able to sufficiently inhibit ribonuclease activity in cells and whole tissues. Because of its biological destructive properties, buffers containing guanidine hydrochloride or guanidine thiocyanate are often called dissociative buffers.
This product is a read-to-use 6M guanidine isothiocyanate solution.
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