English
中文
CAS:201860-17-5
Appearance:Yellow to orange soild
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥98%
Manual Download
Mitochondrial green fluorescence probe is a kind of mitochondrial green fluorescence dye, which can stain and label mitochondria at the nanomolar level. The dye is permeable to the plasma membrane, and it accumulates on the mitochondrial membrane to show bright fluorescence. The localization of the dye in mitochondria does not depend on the mitochondrial membrane potential, and the signal-to-noise ratio of stained fixed cells is not ideal, and the fluorescence signal will be weakened or lost after the cell is fixed and permeated, so we recommend that it only be used to dye living cells. Ex/Em: 490/523 nm.
Usage (for reference only)
Preparation of storage solution A 1 mM mitochondrial green fluorescence probe reserve solution was prepared with DMSO. eg: 50 μg mitochondrial green fluorescence probe dissolved in 74.419 μL DMSO. Note:
The mitochondrial green fluorescence probe reserve solution is recommended to be stored away from light at -20℃ or -80℃ after packaging to avoid repeated freezing and thawing.
Preparation of working fluid
The mitochondrial green fluorescence probe working solution of 20-200 nM was prepared by diluting the storage solution with preheated serum-free cell medium or PBS at 1:500 to 1:50000.
Note:
Please adjust the concentration of the working solution of the mitochondrial green fluorescence probe according to the experimental requirements and pre-experimental results. It is recommended to use the working solution on the spot.
Cell staining
1. Suspension cells
1) The cells were collected by centrifugation and washed twice with PBS for 5 minutes each time. (Generally, the cell density is about 1×106/mL)
2) Add 1 mL of mitochondrial green fluorescence probe working solution and incubate at room temperature for 15-45 minutes.
3) Centrifuge 400 g for 3-4 minutes and discard the supernatant.
4) Add PBS to wash cells twice for 5 minutes each time.
5) Cells were re-suspended with 1 mL serum-free medium or PBS
6) Make observations.
2. Adherent cells
1) The adherent cells were cultured on a sterile cover slide.
2) Remove the cover glass from the medium and absorb the excess medium.
3) Add 100 μL mitochondrial green fluorescent probe working solution, gently shake it to completely cover the cells, and incubate for 15-45 minutes.
4) The working solution of the mitochondrial green fluorescent probe was sucked and washed in the medium 2-3 times for 5 minutes each time. 5) Make observations.
Matters needing attention
1. This product is only used for scientific research by professionals, shall not be used for clinical diagnosis or treatment, and shall not be used for food or medicine.
2. For your safety and health, please wear a lab coat and disposable gloves.
