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CAS:905-96-4
Appearance:Soild
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥98%
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DiOC2(3) emits green fluorescence in the cell. Due to the higher membrane potential, the dye molecules will self-polymerize, so that the fluorescence of the dye will migrate to the red emission wavelength, and the red fluorescence intensity will increase, and the red/green fluorescence signal ratio will be higher. Proton ionophores, such as CCCP, destroy the mitochondrial membrane potential by removing the proton gradient, resulting in a decrease in red fluorescence intensity and a lower red/green fluorescence signal ratio. The excitation and emission wavelengths of DiOC2(3) are about 482nm and 497nm. DiOC2(3) labeled cells were analyzed by flow cytometry (excited at 488nm and detected with FITC(green) and Texas Red(red) emission filters, respectively). DiOC2(3) is a widely used membrane potential probe. For example, when used in the study of mitochondrial membrane potential, DiOC2(3) can penetrate the cytoplasm of eukaryotic cells, and when the concentration is less than 100 nM, the dye accumulates in the mitochondria with active membrane potential, forming red fluorescent aggregates. When the cells are treated with a mitochondrial membrane potential destroying (such as CCCP), the staining intensity of DiOC2(3) is reduced. DiOC2(3) is a membrane potential slow response probe, which is often used to detect the membrane potential of bacteria. DiOC2(3) originally emits green fluorescence, when the membrane voltage increases, the dye molecules enrich on the membrane surface, the optical properties change, emit red fluorescence, using this phenomenon, corresponding to the corresponding proportion, the membrane voltage change can be measured.
