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Your Position: Home > Small Molecule Compounds > Dye & Probe > 10-Acetyl-3,7-dihydroxypenoxazin
10-Acetyl-3,7-dihydroxypenoxazin

CAS:119171-73-2

Appearance:Light brown to brown soild

Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months

Purity:≥98%

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10-Acetyl-3,7-dihydroxypenoxazin
Cat.No：
IA8110
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
IA8110-25mg Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

10-acetyl-3, 7-Dihydroxypenoxazin (ADHP) is a fluorescent probe that can be used to detect peroxidase substrates and is equivalent to AmplexRed, with which it is chemically identical. It is a sensitive fluorescent peroxidase substrate with a low background. In the presence of horseradish peroxidase (HRP), the ADHP reaction produces the intense red fluorescent substance Resorufin. Resorufin has a maximum excitation wavelength of 571nm and a maximum emission wavelength of 585nm, and has a strong visible absorption at the excitation wavelength, which can be detected by a spectrophotometer or a Microplate Reader with an absorbance of 570nm. Unlike other HRP substrates such as dihydrofluorescein and dihydrorhodamine, ADHP is a sensitive and stable fluorescent probe for the detection of HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, since H2O2 is produced in many enzymatic REDOX reactions, ADHP can be used for coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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