Determination of Significance：

Arginine is a semi-essential amino acid in human body and animal body, and plays an important physiological role in protein synthesis and metabolism, as well as the synthesis of polyamine and NO in the organism. Arginine has the effect of lowering blood pressure, in vivo arginine can be decomposed into nitric oxide, nitric oxide can relax the smooth muscle of the blood vessel wall, regulate the elasticity of blood vessels, and have a restorative effect on the endothelium. Arginine can stimulate and induce the secretion of adrenal hormones, thus lowering blood sugar and reducing the production of fatty acids in the body, which can bring down the blood sugar of hyperglycemic patients to normal level.

Measurement Principle：

Arginine content is calculated from its characteristic absorption peak at 525 nm in alkaline medium with cresol and sodium hypochlorite to produce a red product.

Self Provided：

Microplate Reader/ Spectrophotometer, cryogenic centrifuge, water bath/constant temperature incubator, vortex mixer, analytical balance, adjustable pipette, micro glass cuvettes/96-well plates, mortar/homogenizer/cell ultrasonic breaker, distilled water, anhydrous ethanol and ice.

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