Determination of Significance:
The increase in protein content in urine indicates a weakened reabsorption of the renal system, which can usually serve as an auxiliary basis for clinical diagnosis of kidney diseases.
Measurement Principle:
Adding protein precipitant and Ponceau dye to the urine sample, the protein dye complex obtained by centrifugation can be dissolved in alkaline solution. The absorbance value at 560 nm can be measured to calculate the protein content of the sample.
Self Provided:
Spectrophotometer/Microplate reader, desk centrifuge, micro glass cuvette/96 well plate, transferpettor, 2 mL EP tube, ice and distilled water.
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