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Determination of Significance：
Nitric Oxide (NO) is a highly unstable biological free radical with small molecule, simple structure. NO is gas at room temperature, slightly soluble in water and fat-soluble, which can diffuse through biomembrane quickly. As a new biological messenger molecule, it plays a role in transmitting signals between cells and within cells. It is widely distributed in various tissues, especially neural tissues. It also plays a very important role in the nervous, circulatory, respiratory, digestive and urogenital systems of the body.
Measurement Principle：
NO is easily oxidized to form NO2- in the body or in aqueous solution. Under acidic conditions, NO2- and Diazonium sulfonamide produce diazo compounds. The compounds could further couple with naphthyl vinyl diamine. The product has a characteristic absorption peak at 550 nm and its absorbance value can be measured to calculate the NO content.
Self Provided：
Spectrophotometer/Microplate reader, desk centrifuge, balance, transferpettor, micro glass cuvette/96 well plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
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