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Determination of Significance：
UDP-glycose flavonoid glycosyltransferase (UFGT) is the last acting enzyme of the shikimate pathway and the first acting enzyme that causes the formation of stable anthocyanins and their conversion from colorless to colored; UFGT is the key enzyme in the process of fruit coloring, which converts unstable anthocyanins to stable anthocyanin.
Measurement Principle：
UFGT catalyzes the formation of UDP and quercetin glycosides from UDPG and quercetin; UDP oxidizes NADH to NAD+ under the action of pyruvate kinase and lactate dehydrogenase, and the rate of NAD+ generation is directly proportional to the content of UDP, which is reflected by the rate of decrease of absorbance at 340 nm.
Self Provided：
Ultraviolet spectrophotometer, cryogenic centrifuge, water bath/incubator, analytical balance, adjustable pipette, 1mL quartz cuvette, mortar/homogenizer, distilled water and ice.
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