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CAS:17817-20-8
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥95%
Appearance:Solid
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FDG is a reagent used to detect beta galactosidase. Colorless and non-fluorescent FDG hydrolyzes to highly fluorescent fluorescein with excellent spectral characteristics (Ex/Em = 492/520 nm), matching the best detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG results in increased fluorescence near 520nm. Alternatively, FDG can also be used to detect beta-galactosidase in a hair color pattern, since the enzyme product (fluorescein) exhibits a large extinction coefficient. FDG has been widely used in fluorescence microscopy and flow cytometry to identify LACZ-positive cells. It is also used to detect the expression of beta-galactosidase in living cells.
Spectral properties (for reference only)
Absorbance (nm) 487
Correction Factor (260 nm) 0.32
Correction Factor (280 nm) 0.35
Excitation (nm) 498
Emission (nm) 517
