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CAS:83104-85-2
Appearance:Solid
Storage:Store powder at -20℃ for 2 years, in solvent(mother liquid) at -20℃ for 1 month, -80℃ for 6 months.
Purity:≥95%
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Quin-2 is able to bind calcium ions tightly; it is similar to EGTA and binds calcium more tightly than magnesium. Its combination with calcium causes dramatic changes in UV absorption and fluorescence. When combined with calcium, the fluorescence wavelength is longer than that without calcium. When excited by two different wavelengths of light, the ratio of fluorescence intensity of the two different wavelengths tells the concentration ratio relationship of the bonded free calcium ions. The concentration of free Quin-2 can be determined, so the concentration of free calcium ions can also be accurately calculated. Quin-2 can be injected into cells to detect moment-to-moment changes in the concentration of calcium ions in the cells. Quin-2 AM is cell permeable and can be used to study living cells.
How to use (for reference only)
Preparative storage solution
Quin-2 AM was diluted with anhydrous DMSO to prepare a 10 mM reserve solution.
Note:
Quin-2 AM storage solution is recommended to be stored away from light at -20℃ or -80℃ after packaging.
Preparation of working fluid
The storage solution was diluted with preheated HBSS and prepared into 1-10 μM Quin-2 AM working solution.
Note:
Please adjust the concentration of Quin-2AM working liquid according to the actual situation, and use now.
Cell staining
Suspension cell
1 Centrifuge the cells and wash them twice with PBS for 5 minutes each time. The cell density was 1×106/mL
2 Add 1 mL Quin-2 AM working solution and incubate at room temperature for 5-30 minutes.
3 400 g, centrifuge for 3-4 minutes, discard the supernatant.
4 Add PBS to wash cells twice for 5 minutes each time.
5 Cells were suspended with 1 mL HBSS and observed using fluorescence microscopy or flow cytometry.
Adherent cell
1 The adherent cells were cultured on a sterile cover slide.
2 Remove the cover glass from the medium and absorb the excess medium.
3 Add 100 μL dye working solution, shake gently to completely cover the cells, incubate for 5-30 minutes.
4. Remove the dye working solution, wash it with the medium 2-3 times for 5 minutes each time, and observe it with a fluorescence microscope.
