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Nitric Oxide Synthase (NOS) Activity Assay kit

BC5680-241212Manual

Cat.No：BC5680 Solarbio

Detection Method：Spectrophotometer

Storage：Store at -20℃，6 months

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Product Information

Name	Nitric Oxide Synthase (NOS) Activity Assay kit
Detection Method	Spectrophotometer
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/48S

Determination of Significance：

Nitric Oxide Synthase (NOS, EC 1.14.13.39) is a kind of enzyme that catalyzes the synthesis of NO from L-arginine in vivo. It mainly exists in vascular smooth muscle cells, macrophages, endothelial cells, nerve cells, liver cells, glomerular membrane cells and other cells. As a cell signal molecule, it plays a very important role in the nervous, immune and cardiovascular systems of the body.

Measurement Principle：

NOS catalyzes L-arginine, molecular oxygen and NADPH to form NO and NADP+. NO is easily oxidized to form NO^2- and NO^3- in aqueous solution. Under acidic conditions, NO^2- and diazonium sulfonamide produce diazo compounds. The compounds could further couple with naphthyl vinyl diamine. The product has a characteristic absorption peak at 550 nm and its absorbance value can be measured to calculate NOS activity.

Self Provided：

Spectrophotometer, centrifuge, balance, transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1mL glass cuvette, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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