Nitric Oxide Synthase (NOS) Activity Typed Assay kit

Determination of Significance：

Nitric oxide synthase (NOS, EC 1.14.13.39, written as total NOS (TNOS) after) is a type of enzyme that catalyzes the synthesis of NO from L-arginine in living organisms. It mainly exists in various cells such as vascular smooth muscle, macrophages, endothelial cells, neurons, liver cells, and glomerular mesangial cells. According to the different dependence of its enzyme activity on calcium ions, it can be divided into structural NOS (constructive NOS, cNOS) and injury induced NOS (inducible NOS, iNOS). The former requires a certain concentration of calcium ions to activate, while the latter does not rely on exogenous calcium ions.

Measurement Principle：

NOS catalyzes L-arginine, molecular oxygen and NADPH to form NO and NADP+. NO is easily oxidized to form NO^2- and NO^3- in aqueous solution. Under acidic conditions, NO^2- and diazonium sulfonamide produce diazo compounds. The compounds could further couple with naphthyl vinyl diamine. The product has a characteristic absorption peak at 550 nm and its absorbance value can be measured to calculate NOS activity.

Self Provided：

Spectrophotometer/Microplate reader, centrifuge, balance, transferpettor, mortar/homogenizer/cell ultrasonic crusher, micro glass cuvette/96 well plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.