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My Cart> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > α-L-Rhamnosidase Activity Assay kit（Acid Conditions）
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | α-L-Rhamnosidase Activity Assay kit（Acid Conditions） |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
α-L-rhamnosidase α(EC 3.2.1.40) catalyzes the hydrolysis of flavonoid glycosides and terpenes such as rutin, hesperidin and naringin at the end of α-L-rhamnosidase. The optimal pH for most α-L-rhamnosidases is in the acidic or near-neutral range. Fungal α-L-rhamnosidases mostly prefer a more acidic environment, with an optimal pH range of 4.0–6.5. On the other hand, bacterial-derived α-L-rhamnosidase mostly prefers a near-neutral or alkaline environment, with an optimal pH range of 5.0–8.0. This kit is suitable for the determination of α-L-rhamnosidase activity in acidic environments.
Measurement Principle：
Under acidic conditions, α-L-rhamnosidase and p-nitrophenol-α-rhamnoside (PNPR) produce yellow p-nitrophenol (PNP), which has a maximum absorption peak at 400 nm. By measuring the rate of increase of PNP at 400 nm, the magnitude of acidic α-L-rhamnosidase activity can be obtained.
Self Provided：
Spectrophotometer/microplate reader, benchtop centrifuge, water bath, adjustable pipette, microglass cuvette/96-well plate, mortar/homogenizer/cell sonicator, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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