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Determination of Significance：
Myeloperoxidase (MPO) is a white blood cell enzyme secreted by activated neutrophils and monocytes/macrophages, mainly present in the phenylalanine blue granules of neutrophils and monocytes/macrophages. When white blood cells are activated, MPO is released into phagocytic vesicles and plasma, inducing oxidative stress and tissue damage under specific conditions, and is a biomarker of systemic inflammation and oxidative stress.
Measurement Principle：
MPO catalyzes the decomposition of H2O2, while oxidizing ortho anisidine to form a colored substance. There is a characteristic absorption peak at 460nm, and the color depth is linearly related to the activity of MPO.
Self Provided：
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogenizer/cell ultrasonic disruptor, ice and distilled water.
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