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Determination of Significance：
Dihydroflavonol-4-reductase (DFR) is a key enzyme in the biosynthetic pathway of phycocyanin, which catalyzes the production of different anthocyanin precursors from dihydroflavonols. Therefore, DFR determines the type and content of anthocyanins in plants, which in turn affects the color of the tissues or organs of plants.
Measurement Principle：
DFR, NADPH and dihydroflavonols react to produce anthocyanin precursors. NADPH has a maximum absorption peak at 340 nm. The magnitude of DFR activity can be obtained by detecting the rate of change of NADPH at 340 nm.
Self Provided：
Ultraviolet Spectrophotometer, water bath, benchtop centrifuge, balance, adjustable pipettes, 1mL quartz cuvette, mortar and homogenizer, anhydrous ethanol (AR), ammonium sulfate (AR), ice and distilled water.
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