Endogenous peroxidase is widely present in some cells and tissues, including red blood cells, kidney and liver tissues. As a result, there will be higher background and even false positive results in the sample detection of HRP detection system. Therefore, cells or tissue samples should be sealed with appropriate peroxidase blocking solution before staining to eliminate the interference of endogenous peroxidase.
The effect of the peroxidase strong sealing solution produced by Solarbio was significantly better than that of conventional hydrogen peroxide solution and inhibitory dosage form sealing solution, which could fully reduce the non-specific background staining and enhance the specific coloring. It can be used to block tissue or cell endogenous peroxidase during immunohistochemistry (IHC), immunocytochemical staining (ICC) and in situ hybridization (ISH), and can also be applied to step masking of tyramide fluorescent multi-labeling.
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