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The tyramide signal amplification (TSA) system can be used to detect low-abundance targets in fluorescence immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) techniques, increasing signal sensitivity by up to 100 times. The TSA fluorescence kit uses horseradish peroxidase (HRP) to directly catalyze the covalent deposition of fluorophobes around the immobilized enzyme. The labeling process is rapid (less than 10min) and the deposition labeling can be directly observed under standard or confocal microscopy. TSA kits can be combined with conventional staining methods for multicolor imaging, or multiple tyramine reactions can be sequentially performed to label different targets on a sample.
The Solarbio Multi-color tyramine Signal Amplification (TSA) series includes two four-color multi-label fluorescence enhancement kits. fluorescein, Cyanine3, Cyanine5 and DAPI can be fluorescein, cyanine3, cyanine4 and DAPI, and three proteins and nuclei can be simultaneously labeled.
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