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Determination of Significance：
The presence of sulfhydryl allows proteins to undergo disulfide bond formation, thereby maintaining molecular stability and functionality. Sulfhydryl is also involved in redox reactions and have important biological roles. In cells, changes in sulfhydryl content are closely related to the onset and progression of a variety of diseases, and thus sulfhydryl have become an important object of study in the biomedical field. This kit determines the sum of sulfhydryl produced by protein disulfide bond breakage and its own free sulfhydryl.
Measurement Principle：
Reducing agent will cause disulfide bond cleavage to generate sulfhydryl, sulfhydryl will undergo nucleophilic reaction, that is, sulfhydryl reacts with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) to produce a yellow color compound, with a maximum absorption peak at 412 nm, from which the total sulfhydryl content of proteins can be calculated.
Self Provided：
Spectrophotometer/microplate reader, tabletop centrifuge, water bath, adjustable pipette, Micro glass cuvettes/96 well plates, acetone, mortar/homogenizer/ sonicator and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
