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Storage:-20℃
Determination of Significance：
"Uridine diphosphate glucuronosyltransferase (UDPGT/UGT, EC 2.4.1.17) is a membrane protein bound to the endoplasmic reticulum that catalyzes the transfer of glucuronic acid groups from the donor uridine diphosphate glucuronic acid to a receptor, including phenols, alcohols, amines, and fatty acids. Glucuronidation metabolism promotes the excretion of drugs and other exogenous substances through the kidneys or bile, and plays an important role in the metabolism, detoxification and clearance of organisms.
Measurement Principle：
UDPGT catalyzes the transfer of the glucuronic acid group of the donor uridine diphosphate glucuronic acid to p-nitrophenol, which has a characteristic absorption peak at 405 nm, and UDPGT activity can be calculated by measuring the rate of reduction in absorbance values.
Self Provided：
Visible spectrophotometer, cryogenic centrifuge, analytical balance, water bath/incubator, 1 mL glass cuvette, adjustable pipette, mortar/homogenizer, ice, and distilled water.
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