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Determination of Significance：
Albumin is the most important protein in human plasma, synthesized by the liver. It is an important nutrient in the body that maintains plasma osmolality and can be combined with a variety of nutrients, hormones and drugs. Albumin content can reflect the nutritional status of the organism, and can also detect diseases that affect the metabolic function of the liver, such as cirrhosis, liver injury, malnutrition, malignant tumors and so on.
Measurement Principle：
Serum albumin is positively charged in pH 4.2 buffer, and in the presence of non-ionic surfactants, it can combine with the negatively charged dye bromocresol green to form a blue-green complex, with an absorption peak at a wavelength of 630 nm, and its color depth is positively proportional to the concentration of albumin.
Self Provided：
Centrifuge, spectrophotometer, bowl/homogenizer/cell ultrasonic breaker, 1mL glass cuvette, ice and distilled water.
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