Glucose Content Assay kit (o-Toluidine Colorimetry)

Determination of Significance：

Glucose is not only the main substrate for cellular energy metabolism, but also its metabolic intermediates are important substrates for biosynthesis. In the case of mammals, glucose is not only the only energy source for the nervous system of the brain, muscles, adipose tissue, etc, but also is closely related to the synthesis of reducing coenzymes, lactose and milk fat.

Measurement Principle：

When glucose and o-toluidine are heated in a strong acid solution, the aldehyde group of glucose condenses with o-toluidine to form glucosylamine, which dehydrates to form Schiff base, which is blue-green in color with an absorption peak at 630nm. The kit is suitable for the determination of animal tissue, serum (plasma) and cell (bacterial) samples.

Self Provided：

Visible spectrophotometer/microplate reader, cryogenic centrifuge, analytical balance, micro glass cuvette/ 96-well plate, mortar/homogenizer/cell ultrasonic breaker, adjustable pipette, acetic acid (AR > 98%), 5% trichloroacetic acid (determine if you need to bring your own depending on precautions), isopropyl alcohol (determine if you need to bring your own depending on precautions), ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.