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Protein Free Thiol Content Assay Kit

BC5890-241212Manual

Cat.No：BC5890 Solarbio

Detection Method：Spectrophotometer

Storage：Store at 2-8℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Protein Free Thiol Content Assay Kit
Detection Method	Spectrophotometer
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	50T/24S

Determination of Significance：

The presence of sulfhydryl allows proteins to undergo disulfide bond formation, thereby maintaining molecular stability and functionality. Sulfhydryl are also involved in redox reactions and have important biological roles. In cells, changes in sulfhydryl content are closely related to the onset and progression of a variety of diseases, and thus sulfhydryl have become an important object of study in the biomedical field. This kit determines the free sulfhydryl content of proteins.

Measurement Principle：

Under certain conditions, sulfhydryl group will undergo nucleophilic reaction, i.e. sulfhydryl group reacts with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) to produce a yellow color compound with a maximum absorption peak at 412 nm, from which the free sulfhydryl content of protein can be calculated.

Self Provided：

Spectrophotometer, tabletop centrifuge, water bath, adjustable pipette, 1 mL glass cuvette, Acetone, mortar/homogenizer/ sonicator and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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