T4 DNA Fast Ligase
Cat.No：T1411 Solarbio
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> Molecular Biology > Molecular Cloning-Related Enzymes > PCR-Associated Enzyme > T4 DNA Fast Ligase
Product Information
| Storage | Store at -20℃ |
| Unit | Bottle |
| Specification | 200U 1KU |
Product introduction
T4 DNA Ligase(Fast) is produced by Escherichia coli harboring the T4 phage gene 30. This enzyme catalyzes the formation of a phosphodiester bond between the 5' -phosphate group and the 3' -hydroxyl group between double-helical DNA or RNA. "This enzyme repairs single-strand breaks between double-stranded DNA, RNA, or DNA/RNA complexes, and can join DNA fragments with sticky or flat ends, but is inactive against single-stranded nucleic acids." It is mainly used for cloning DNA fragments of restriction enzyme products, site-directed mutagenesis and PCR product cloning, linear DNA self-looping and repairing double-stranded DNA defects. T4 DNALigase(Fast) requires ATP as a cofactor and takes only 10 min to complete the cohesive end joining reaction at room temperature.
Unit definition
The enzyme of 1 Weiss unit catalyzed the conversion of 1 nmol of [32 PPi] to the activated carbon adsorbed state in 20 min at 37 ° C. One Weiss unit is equivalent to approximately 200 sticky end joining reaction units (CEU), which is equivalent to ligation of a 50% HindiII-digested λDNA fragment in 30 min at 16 ° C.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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