QuickCut Fast restriction endonuclease Sfil

Recognition site

GGCCNNNNNGGCC

5 '... G G C C N N N N ↓ N G G C C... 3 '

3 '... C C G G N ↑ N N N N C C G G... 5 '

Product introduction

LightNing fast endonuclease is a series of genetically engineered fast restriction endonuclease, which is suitable for fast enzyme cutting of plasmid DNA, PCR products or genomic DNA. All LightNing fast endonuclease enzymes have excellent activity in the universal CutOne or CutOne Color Buffer, and can complete the enzyme digestion in 5 to 15 minutes. In addition, the dephosphorylation and linking reagents are 100% active in the CutOne Buffer, supporting a tubeization reaction and improving the enzyme-reduction-modified-linking experience.

The CutOne Color Buffer includes red and yellow tracer dyes that allow the product to be used directly for gel electrophoresis. The migration rate of the red dye in CutOne Color Buffer was close to that of 2500 bp double-stranded DNA fragment in 1% agarose gel. The migration rate of yellow dye and 10 bp double-stranded DNA fragment in 1% agarose gel is close.

Features and usage

Fast endonuclide, the reaction can be completed within 5 to 15 minutes

Suggested reaction condition

1× CutOne buffer;

Incubation at 50℃;

The reaction system was formulated according to DNA Rapid enzyme digestion Process.

Inactivation condition

Do not heat inactivation, please use phenol chloroform extraction or column purification.

Methylation sensitivity

For DNA methylated by CpG, the shear may be blocked,

For DNA methylated by Dcm, the shear may be blocked.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.