Restriction endonuclease BspQI
Cat.No：Q2540 Solarbio
Storage：Store at -20℃，2 years.
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Molecular Cloning-Related Enzymes > Endonuclease > Restriction endonuclease BspQI
Product Information
| Name | Restriction endonuclease BspQI |
| Storage | Store at -20℃，2 years. |
| Grade | 10U/ul |
| Unit | Piece |
| Specification | 500U |
Isomerase
SapI, LguI, PciSI
Note: Isoschizase may have different sensitivities to different methylation modifications.
Recognition site
GCTCTTC(1/4)
5 '... G C T C T T C (N)1↓... 3 '
3 '... C G A G A A G (N)4↑... 5 '
Product introduction
BspQI is a Type IIS restriction enzyme that recognizes non-palindromic sequences and cuts them outside of the recognition sequence, and is commonly used in Golden Gate assembly. The optimized reaction Buffer maximizes the function of BspQI, while the reaction buffer contains recombinant albumin, which enhances the stability of multiple enzymes.
Features and usage
Suggested reaction condition
1× HN buffer;
Incubation at 50℃;
The reaction system was formulated according to DNA enzyme digestion process.
Inactivation condition
Incubate at 80℃for 20 min.
Methylation sensitivity
They are insensitive to Dam methylation, insensitive to Dcm methylation, and insensitive to CpG methylation.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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