Recognition site

m?CNNG(9/13)

5 '... m? C N N G(N)? ↓... 3 '

3 '... G N N C(N)?? ↑... 5 '*

*SgeI can identify and cut DNA target sequences containing 5-mC sites, one strand or double strand methylation can be recognized.

Product introduction

SgeI can cut DNA targets containing 5-methylcytosine on single or double-stranded DNA. The SgeI restriction enzyme recognizes the m?CNNG(9/13)^ site and cuts best in its unique buffer at 37°C. To ensure consistent performance, the enzyme Storage Buffer contains pre-mixed BSA, which enhances the stability of the enzyme and combines with contaminants that may be present in the DNA preparation.

Features and usage

Suggested reaction condition

1× Sgel buffer;

Incubation at 37℃;

The reaction system was formulated according to DNA enzyme digestion process.

Inactivation condition

Incubate at 80℃for 20 min.

Methylation sensitivity

They are insensitive to Dam methylation, insensitive to Dcm methylation, and insensitive to CpG methylation.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.