Restriction enzyme Sgel
Cat.No：Q2560 Solarbio
Storage：Store at -20℃，2 years.
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> Molecular Biology > Molecular Cloning-Related Enzymes > Endonuclease > Restriction enzyme Sgel
Product Information
| Name | Restriction enzyme Sgel |
| Storage | Store at -20℃，2 years. |
| Grade | 5U/ul |
| Unit | Piece |
| Specification | 250U |
Recognition site
m?CNNG(9/13)
5 '... m? C N N G(N)? ↓... 3 '
3 '... G N N C(N)?? ↑... 5 '*
*SgeI can identify and cut DNA target sequences containing 5-mC sites, one strand or double strand methylation can be recognized.
Product introduction
SgeI can cut DNA targets containing 5-methylcytosine on single or double-stranded DNA. The SgeI restriction enzyme recognizes the m?CNNG(9/13)^ site and cuts best in its unique buffer at 37°C. To ensure consistent performance, the enzyme Storage Buffer contains pre-mixed BSA, which enhances the stability of the enzyme and combines with contaminants that may be present in the DNA preparation.
Features and usage
Suggested reaction condition
1× Sgel buffer;
Incubation at 37℃;
The reaction system was formulated according to DNA enzyme digestion process.
Inactivation condition
Incubate at 80℃for 20 min.
Methylation sensitivity
They are insensitive to Dam methylation, insensitive to Dcm methylation, and insensitive to CpG methylation.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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